Primer

Part:BBa_K5406003

Designed by: Kristian Verdhi   Group: iGEM24_Athens   (2024-09-12)


Reverse Primer for PPKTe Sequencing

After cloning Poly-Phosphatate Kinase (PPK), it was required that it would sequenced to verify an appropriate cloning. For that, primers needed to be desinged.

Following are some data on the primer pair:

Sequence (5'->3') / Template strand / Tm / GC% / Self complementarity / Self 3'complementarity

TAACAAGCAGGAGCAGACCG / Plus / 60.04 / 55.00 / 2.00 / 2.00

TGCCATCAGCACGGAATGAG / Minus / 60.75 / 55.00 / 3.00 / 0.00

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None